To connect to the group data drive from anywhere in the world, you just need to do a few steps: 1) Go to https://people.chem.umass.edu/wiki/index.php?title=VPN and download the Tunnelblick client that fits your OS. Follow the instructions on that page (simple) for installation and setup. 2) Be sure you are all set up with an LDAP [...]
Working with restriction enzymes Move the vial of restricion enzyme you need into an empty, frozen (blue) carry box and bring it to your bench. Quickly spin (5 seconds) the enzyme tube to be sure none is in the cap. Remove the amount of enzyme you need for your digest. Tighten the cap to be [...]
NMR solvent info TLC stains P-anisaldehyde Particularly good for nucleophilic compounds. 15 mL AcOH 3.5 mL p-anisaldehyde 350 mL of ice cold EtOH 50 mL H2SO4, slowly over 60 min Store at -20. Ninhydrin Good for aimno acids. 1.5 g ninhydrin 100mL n-Butanol 3 mL AcOH KMnO4 Good for oxidizable compounds. 1.5 g KMnO4 10 [...]
Basic Linux commands: command what it does… cd change directory cd .. go up one directory cd ../.. go up two directories ls list files in current directory ls -lart list files in current directory in reverse chrom order mv file1 file2 (change name of file1 to file2) cp file1 file2 (make a copy of [...]